Coding

Part:BBa_K2799000:Design

Designed by: Jacob Mejlsted, Joen Haahr Jensen, David Faurdal   Group: iGEM18_DTU-Denmark   (2018-10-07)


melA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 379
    Illegal NheI site found at 517
    Illegal NheI site found at 1243
    Illegal NheI site found at 1486
    Illegal NheI site found at 1537
    Illegal NotI site found at 1709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 28
    Illegal XhoI site found at 79
    Illegal XhoI site found at 949
    Illegal XhoI site found at 1804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to aggresive codon optimizing, regions of repeats can complicate the de novo synthesis of this gene.


Source

Codon optimized for Aspergillus oryzae. Protein sequence from Rhizobium etli CFN42. See BBa_K193600

References